Lias^tm2Mae
Targeted Allele Detail

Summary

• Symbol: Lias^tm2Mae
• Name: lipoic acid synthetase; targeted mutation 2, Nobuyo Maeda
• MGI ID: MGI:5810125
• Synonyms: Lias^High
• Gene: Lias Location: Chr5:65548840-65567766 bp, + strand Genetic Position: Chr5, 33.66 cM, cytoband C3.3
• Alliance: Lias^tm2Mae page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:236908
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The targeting vector consists of a loxP site, the 3' UTR sequence of the bovine growth hormone gene, a neomycin cassette and a loxP site followed by the 3'-UTR sequence of the cFos gene. The bovine growth hormone gene 3'-UTR sequence directs expression of the endogenous Lias creating a hypermorph allele. Expression is 150% of endogenous gene expression. (J:236908)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lias Mutation: 26 strains or lines available

Notes

ES cell line - Chemicon B/6N

References

• Original: J:236908 Xu L, et al., Influence of Different Levels of Lipoic Acid Synthase Gene Expression on Diabetic Nephropathy. PLoS One. 2016;11(10):e0163208
• All: 1 reference(s)