Apoe^{tm1.1(APOE*4)Adiuj}
Targeted Allele Detail

Summary

• Symbol: Apoe^{tm1.1(APOE*4)Adiuj}
• Name: apolipoprotein E; targeted mutation 1.1, MODEL-AD Center
• MGI ID: MGI:5810209
• Synonyms: APOE^epsilon4
• Gene: Apoe Location: Chr7:19430034-19433113 bp, - strand Genetic Position: Chr7, 9.94 cM
• Alliance: Apoe^{tm1.1(APOE*4)Adiuj} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:323137
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion, Nucleotide substitutions
• Apoe^{tm1.1(APOE*4)Adiuj} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APOE (348)

  Apoe	(MGI:88057)

  APOE4 isoform

• Mutation details: The targeting vector contains a 3' FRT flanked neo cassette and 1.5 kb of human APOE gene sequence including exons 2, 3, 4 and a portion of the 3'UTR sequence (the APOE4 isoform) which is used to replace exons 2, 3 and most of exon 4 of the endogenous sequence. The human APOE sequence carries a 2 nucleotide A-to-T substitution in intron 3. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:323137)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 39 strains available Cell Lines: 0 lines available
• Carrying any Apoe Mutation: 158 strains or lines available

References

• Original: J:323137 Foley KE, et al., The APOE (epsilon3/epsilon4) Genotype Drives Distinct Gene Signatures in the Cortex of Young Mice. Front Aging Neurosci. 2022;14:838436
• All: 39 reference(s)