Arid3b^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Arid3b^{tm1c(KOMP)Wtsi}
• Name: AT-rich interaction domain 3B; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5812005
• Synonyms: Arid3b^fl
• Gene: Arid3b Location: Chr9:57697636-57744076 bp, - strand Genetic Position: Chr9, 31.56 cM, cytoband C
• Alliance: Arid3b^{tm1c(KOMP)Wtsi} page
• IMPC: Arid3b gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:236980
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 57705103 of Chromosome 9 upstream of exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 4 at position 57705960. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exon 4. (J:236980)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Arid3b Mutation: 45 strains or lines available

References

• Original: J:236980 Kurkewich JL, et al., Arid3b Is Critical for B Lymphocyte Development. PLoS One. 2016;11(8):e0161468
• All: 1 reference(s)