Dhps^{tm2c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dhps^{tm2c(EUCOMM)Wtsi}
• Name: deoxyhypusine synthase; targeted mutation 2c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5812110
• Synonyms: Dhs^p
• Gene: Dhps Location: Chr8:85798386-85801791 bp, + strand Genetic Position: Chr8, 41.55 cM
• Alliance: Dhps^{tm2c(EUCOMM)Wtsi} page
• IMPC: Dhps gene page

Mutation origin

• Mutant Cell Lines: EPD0628_1_B06, EPD0628_1_C06, EPD0628_1_F05
• Germline Transmission: Earliest citation of germline transmission: J:236582
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 85798794 of Chromosome 8 upstream of exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 7 at position 85801230. Exons 2-7 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exons 2-7. (J:236582)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dhps Mutation: 21 strains or lines available

References

• Original: J:236582 Pallmann N, et al., Biological Relevance and Therapeutic Potential of the Hypusine Modification System. J Biol Chem. 2015 Jul 24;290(30):18343-60
• All: 3 reference(s)