Pofut2^tm2.2Bch
Targeted Allele Detail

Summary

• Symbol: Pofut2^tm2.2Bch
• Name: protein O-fucosyltransferase 2; targeted mutation 2.2, Bernadette C Holdener
• MGI ID: MGI:5812116
• Synonyms: Pofut2-LoxP
• Gene: Pofut2 Location: Chr10:77095052-77105409 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Pofut2^tm2.2Bch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:234992
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 3 and 4 of the targeted gene were replaced with a construct containing: loxP-FRT-polyA-neomycin (in reverse transcriptional orientation)-FRT-exons 3-9 of Pofut2 mini cDNA-polyA-loxP-Stop-mCherry-polyA. Flp-mediated recombination removed the FRT-flanked neo cassette. mCherry fluorescence is not detectable in this allele. Cre-mediated recombination removed the loxP-flanked exons 3-9. In-situ hybridization and H&E staining indicates that embryos fail to express markers of extra-embryonic ectoderm and mesoderm differentiation. (J:234992)

Expression

In Mice Carrying this Mutation:	38 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pofut2 Mutation: 29 strains or lines available

References

• Original: J:234992 Benz BA, et al., Genetic and biochemical evidence that gastrulation defects in Pofut2 mutants result from defects in ADAMTS9 secretion. Dev Biol. 2016 Aug 1;416(1):111-22
• All: 2 reference(s)