Malt1^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Malt1^{tm1c(EUCOMM)Hmgu}
• Name: MALT1 paracaspase; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5812177
• Synonyms: MALT1^F, Malt1^fl
• Gene: Malt1 Location: Chr18:65564010-65612138 bp, + strand Genetic Position: Chr18, 38.56 cM
• Alliance: Malt1^{tm1c(EUCOMM)Hmgu} page
• IMPC: Malt1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:237235
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 65577475 of Chromosome 18 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 65578310. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:237235)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Malt1 Mutation: 35 strains or lines available

Notes

Mutant cell line HEPD0618_3_D10 was used in J:333424 to create mutant mice which demonstrated germline transmission.

References

• Original: J:237235 Drennan MB, et al., NKT sublineage specification and survival requires the ubiquitin-modifying enzyme TNFAIP3/A20. J Exp Med. 2016 Sep 19;213(10):1973-81
• All: 8 reference(s)