Gt(ROSA)26Sor^tm2(ATF4)Myz
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm2(ATF4)Myz
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Makoto Miyazaki
• MGI ID: MGI:5812282
• Synonyms: Rosa26-ATF4^loxtg
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm2(ATF4)Myz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:237331
• Parent Cell Line: EC7.1 (ES Cell)
• Strain of Origin: (C57BL/6J x 129X1/SvJ)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^tm2(ATF4)Myz expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ATF4 (468)

  Atf4	(MGI:88096)

• Mutation details: The targeting vector containing a loxP site, PGK-puromycin-pA cassette (in reverse transcriptional orientation), 4x SV40-pA STOP, loxP, and FLAG-human ATF4-WPRE-BGH-pA cassette sequence is introduced to the Gt(ROSA)26Sor gene via a FLP recombinase-mediated cassette exchange method. (J:237331)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:237331 Masuda M, et al., Activating transcription factor-4 promotes mineralization in vascular smooth muscle cells. JCI Insight. 2016 Nov 03;1(18):e88646
• All: 4 reference(s)