Hnrnpa3^em1Ionsz
Endonuclease-mediated Allele Detail

Summary

• Symbol: Hnrnpa3^em1Ionsz
• Name: heterogeneous nuclear ribonucleoprotein A3; endonuclease-mediated mutation 1, Qiang Sun
• MGI ID: MGI:5812384
• Synonyms: Hnrnpa3a KO
• Gene: Hnrnpa3 Location: Chr2:75489605-75499751 bp, + strand Genetic Position: Chr2, 44.75 cM, cytoband A7.2
• Alliance: Hnrnpa3^em1Ionsz page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Intragenic deletion
• Mutation details: The CRISPR/Cas9 system was used to generate a 25-bp deletion in exon 1. Exon 1 is present in the longer Hnrnpa3a protein isoform but absent in the shorter Hnrnpa3b isoform. Immunoblot analysis revealed a marked reduction in the level of Hnrnpa3a, but not Hnrnpa3b, in the brain of homozygous mutant mice at E14.5. Immunostaining revealed that protein levels were barely detectable in the ventricular zone (VZ) and subventricular zone (SVZ) regions at E13.5. (J:237332, J:288307)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hnrnpa3 Mutation: 22 strains or lines available

References

• Original: J:237332 Li Y, Direct data submission for alleles of Trim60, Zscan5b, Icam5, Lrp3, and Hnrnpa3. MGI Direct Data Submission. 2016
• All: 2 reference(s)