Spata13^tm1Taki
Targeted Allele Detail

Summary

• Symbol: Spata13^tm1Taki
• Name: spermatogenesis associated 13; targeted mutation 1, Tetsu Akiyama
• MGI ID: MGI:5816603
• Synonyms: Asef2^-
• Gene: Spata13 Location: Chr14:60871450-61002005 bp, + strand Genetic Position: Chr14, 31.77 cM
• Alliance: Spata13^tm1Taki page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:157023
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: LacZ and neomycin resistance genes were inserted in-frame at codon 99 of the gene. Northern blot analysis of genomic DNA using external and neomycin probes revealed that the gene is correctly targeted and immunoblot analysis confirmed loss of expression of the endogenous protein. (J:157023)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spata13 Mutation: 79 strains or lines available

References

• Original: J:157023 Kawasaki Y, et al., The adenomatous polyposis coli-associated exchange factors Asef and Asef2 are required for adenoma formation in Apc(Min/+)mice. EMBO Rep. 2009 Dec;10(12):1355-62
• All: 2 reference(s)