Blvra^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Blvra^{tm1c(EUCOMM)Hmgu}
• Name: biliverdin reductase A; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5816874
• Gene: Blvra Location: Chr2:126912585-126939004 bp, + strand Genetic Position: Chr2, 61.76 cM, cytoband F3
• Alliance: Blvra^{tm1c(EUCOMM)Hmgu} page
• IMPC: Blvra gene page

Mutation origin

• Mutant Cell Line: HEPD0510_3_B01
• Germline Transmission: Earliest citation of germline transmission: J:237631
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 126924416 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 126925207. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:237631)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Blvra Mutation: 34 strains or lines available

References

• Original: J:237631 Hinds TD Jr, et al., Biliverdin Reductase A Attenuates Hepatic Steatosis by Inhibition of Glycogen Synthase Kinase (GSK) 3beta Phosphorylation of Serine 73 of Peroxisome Proliferator-activated Receptor (PPAR) alpha. J Biol Chem. 2016 Nov 25;291(48):25179-25191
• All: 2 reference(s)