Vipas39^{tm1c(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Vipas39^{tm1c(KOMP)Mbp}
• Name: VPS33B interacting protein, apical-basolateral polarity regulator, spe-39 homolog; targeted mutation 1c, Mouse Biology Program, UC Davis
• MGI ID: MGI:5816979
• Synonyms: Vipas39^fl
• Gene: Vipas39 Location: Chr12:87285642-87313030 bp, - strand Genetic Position: Chr12, 41.44 cM
• Alliance: Vipas39^{tm1c(KOMP)Mbp} page
• IMPC: Vipas39 gene page

Abnormal collagen structure in Vipas39^{tm1c(KOMP)Mbp}/Vipas39^{tm1c(KOMP)Mbp} Gt(ROSA)26Sor^{tm1(cre/ERT2)Tyj}/Gt(ROSA)26Sor⁺, and Vps33b^tm1.1Arte/Vps33b^tm1.1Arte Gt(ROSA)26Sor^{tm1(cre/ERT2)Tyj}/Gt(ROSA)26Sor⁺ mouse tail tendon

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:236095
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 87298249 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 87299141. The critical exon 10 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exon 10. (J:236095)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vipas39 Mutation: 31 strains or lines available

References

• Original: J:236095 Banushi B, et al., Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis. Nat Commun. 2016 Jul 20;7:12111
• All: 2 reference(s)