Cgref1^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cgref1^em1(IMPC)J
• Name: cell growth regulator with EF hand domain 1; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:5817761
• Synonyms: Cgref1^em1J
• Gene: Cgref1 Location: Chr5:31090487-31102771 bp, - strand Genetic Position: Chr5, 16.9 cM
• Alliance: Cgref1^em1(IMPC)J page
• IMPC: Cgref1 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele from project Cgref1-8199J-F4208 was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences GCCCAGTGTGAAGCATCAGA, TTAGAATAAGACCTGACACT, GGAGGACTATGAATACCCAT and GAATGGTTCCACATGCAAGG, which resulted in a 489 bp deletion beginning at Chromosome 5 negative strand position 30,936,119 bp, GTATTCATAGTCCTCCCTGA, and ending after AGAATAAGACCTGACACTGG at 30,935,631 bp (GRCm38/mm10). This mutation deletes exon 2 and 386 bp of flanking intronic sequence including the splice acceptor and donor, and is predicted to cause a change of amino acid sequence after residue 39 and early truncation 31 amino acids later. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Cgref1 Mutation: 15 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)