Lefty1^{tm1.1(cre/Esr1*)Sia}
Targeted Allele Detail

Summary

• Symbol: Lefty1^{tm1.1(cre/Esr1*)Sia}
• Name: left right determination factor 1; targeted mutation 1.1, Shinich Aizawa
• MGI ID: MGI:5817777
• Gene: Lefty1 Location: Chr1:180762587-180765965 bp, + strand Genetic Position: Chr1, 84.46 cM, cytoband F
• Alliance: Lefty1^{tm1.1(cre/Esr1*)Sia} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:222398
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Lefty1^{tm1.1(cre/Esr1*)Sia} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Lefty1 (MGI:107405)

• Mutation details: The targeting vectors were constructed with the RP23-230B21 BAC clone. A CreERTM fusion protein carrying a polyA sequence and an FRT-flanked PGK-Neo cassette are inserted into the first exon of Lefty1 gene. FLPe-mediated recombination excised the PGK-Neo cassette, leaving a single FRT site behind. (J:222398)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Lefty1 (mouse) Summary of all recombinase alleles driven by Lefty1. Alliance mouse Lefty1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lefty1 Mutation: 41 strains or lines available

References

• Original: J:222398 Hoshino H, et al., AVE protein expression and visceral endoderm cell behavior during anterior-posterior axis formation in mouse embryos: Asymmetry in OTX2 and DKK1 expression. Dev Biol. 2015 Jun 15;402(2):175-91
• All: 1 reference(s)