Gt(ROSA)26Sor^{tm2(CAG-NuTRAP)Evdr}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(CAG-NuTRAP)Evdr}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Evan Rosen
• MGI ID: MGI:5817820
• Synonyms: Gt(ROSA)26Sor^{tm1(CAG-birA,-mCherry/Rangap1,-EGFP/Rpl10a)Evdr}, NuTRAP, RANGAP-TRAP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(CAG-NuTRAP)Evdr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:347707
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector was designed with (from 5' to 3') with a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, a FRT site, a loxP-flanked STOP cassette, the E. Coli biotin ligase gene (birA), a 2A peptide, the BLRP-mCherry-mRANGAP1 fusion gene (described below), a 2A peptide, the EGFP-L10a fusion protein (an enhanced green fluorescent protein gene [EGFP] fused in-frame to the N-terminus of a cDNA sequence encoding the mouse 60S ribosomal subunit L10a [Rpl10a]), a WPRE, a polyA signal, an AttB site, a PGK-FRT-Neo-PGK polyA, and an AttP site. This entire targeting vector was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. The BLRP-mCherry-mRANGAP1 fusion gene consists of a monomeric red fluorescent protein sequence fused in-frame to the N-terminal of a cDNA sequence encoding the mouse nuclear membrane RAN GTPase activating protein 1. This fusion gene is tagged at the mCherry N-terminal with a biotin ligase recognition peptide (BLRP ; a 23 amino acid protein with AviTag core. (J:101977, J:347707)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1040 strains or lines available

References

• Original: J:347707 Roh HC, et al., Simultaneous Transcriptional and Epigenomic Profiling from Specific Cell Types within Heterogeneous Tissues In Vivo. Cell Rep. 2017 Jan 24;18(4):1048-1061
• All: 20 reference(s)