Gpr88^tm1.2Ics
Targeted Allele Detail

Summary

• Symbol: Gpr88^tm1.2Ics
• Name: G-protein coupled receptor 88; targeted mutation 1.2, Mouse Clinical Institute
• MGI ID: MGI:5818030
• Synonyms: Gpr88^-
• Gene: Gpr88 Location: Chr3:116043304-116047123 bp, - strand Genetic Position: Chr3, 50.23 cM, cytoband G1
• Alliance: Gpr88^tm1.2Ics page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:236208
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 2 was flanked by loxP sites and an FRT site flanked neomycin resistance gene cassette with an additional loxP site was inserted downstream in intron 2. Flp-mediated recombination removed the neo cassette. Exon 2 was deleted through Cre-mediated recombination, creating a knock-out allele. In situ hybridization analysis confirmed the absence of transcript in homozygous mutant brains. Furthermore, GPR88-mediated [35S]-GTPgammaS binding was abolished in the striatum, demonstrating lack of GPR88 receptor signaling in mutant mice. (J:236208)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gpr88 Mutation: 16 strains or lines available

References

• Original: J:236208 Meirsman AC, et al., Mice Lacking GPR88 Show Motor Deficit, Improved Spatial Learning, and Low Anxiety Reversed by Delta Opioid Antagonist. Biol Psychiatry. 2016 Jun 1;79(11):917-27
• All: 6 reference(s)