Myh6^{tm1.1(cre/Esr1*)Ccai}
Targeted Allele Detail

Summary

• Symbol: Myh6^{tm1.1(cre/Esr1*)Ccai}
• Name: myosin, heavy polypeptide 6, cardiac muscle, alpha; targeted mutation 1.1, Chen-Leng Cai
• MGI ID: MGI:5818369
• Synonyms: Myh6^MerCreMer
• Gene: Myh6 Location: Chr14:55179378-55204384 bp, - strand Genetic Position: Chr14, 28.01 cM
• Alliance: Myh6^{tm1.1(cre/Esr1*)Ccai} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:233991
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Myh6^{tm1.1(cre/Esr1*)Ccai} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Myh6 (MGI:97255)

• Mutation details: A cassette encoding cre recombinase fused to 2 mutated murine estrogen receptor ligand-binding domains (Mer; Esr1*) followed by FRT flanked neo selection cassette are inserted into 6 bp upstream of the start codon of Myh6 locus, with disruption of endogenous ATG by homologous recombination in ES cells. Flp-mediated recombination removed the selection cassette, leaving a single FRT site behind. (J:233991)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Myh6 (mouse) Summary of all recombinase alleles driven by Myh6. Alliance mouse Myh6 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Myh6 Mutation: 206 strains or lines available

References

• Original: J:233991 Yan J, et al., A Murine Myh6MerCreMer Knock-In Allele Specifically Mediates Temporal Genetic Deletion in Cardiomyocytes after Tamoxifen Induction. PLoS One. 2015;10(7):e0133472
• All: 2 reference(s)