Kit^{tm1(cre/Esr1*)Kkar}
Targeted Allele Detail

Summary

• Symbol: Kit^{tm1(cre/Esr1*)Kkar}
• Name: KIT proto-oncogene receptor tyrosine kinase; targeted mutation 1, Klaus Karjalainen
• MGI ID: MGI:5818476
• Synonyms: Kit^MercreMer
• Gene: Kit Location: Chr5:75735647-75817382 bp, + strand Genetic Position: Chr5, 39.55 cM
• Alliance: Kit^{tm1(cre/Esr1*)Kkar} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:233387
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: BALB/c

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Kit^{tm1(cre/Esr1*)Kkar} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Kit (MGI:96677)

• Mutation details: A codon-optimized IRES-MercreMer (MercreMer: cre recombinase fused to two modified estrogen receptor ligand binding domains at both ends) fused gene and a frt-flanked neomycin selection cassette were inserted into the 3' untranslated region (20 bp after the stop codon) of the Kit gene in embryonic stem cells by homologous recomibination. (J:233387)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Kit (mouse) Summary of all recombinase alleles driven by Kit. Alliance mouse Kit gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kit Mutation: 182 strains or lines available

References

• Original: J:233387 Sheng J, et al., Most Tissue-Resident Macrophages Except Microglia Are Derived from Fetal Hematopoietic Stem Cells. Immunity. 2015 Aug 18;43(2):382-93
• All: 8 reference(s)