Kit^{tm3.1(cre/Esr1*)Ccai}
Targeted Allele Detail

Summary

• Symbol: Kit^{tm3.1(cre/Esr1*)Ccai}
• Name: KIT proto-oncogene receptor tyrosine kinase; targeted mutation 3.1, Chen-Leng Cai
• MGI ID: MGI:5818536
• Synonyms: c-kit^MerCreMer
• Gene: Kit Location: Chr5:75735647-75817382 bp, + strand Genetic Position: Chr5, 39.55 cM
• Alliance: Kit^{tm3.1(cre/Esr1*)Ccai} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:227907
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Kit^{tm3.1(cre/Esr1*)Ccai} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Kit (MGI:96677)

• Mutation details: A cassette encoding cre recombinase fused to 2 mutated murine estrogen receptor ligand-binding domains (Mer; Esr1*) followed by FRT flanked neo selection cassette are inserted into the start codon of kit locus with disruption of endogenous ATG by homologous recombination in ES cells. Flp-mediated recombination removed the neo selection cassette, leaving a single FRT site behind. (J:227907)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Kit (mouse) Summary of all recombinase alleles driven by Kit. Alliance mouse Kit gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kit Mutation: 182 strains or lines available

References

• Original: J:227907 Sultana N, et al., Resident c-kit(+) cells in the heart are not cardiac stem cells. Nat Commun. 2015;6:8701
• All: 1 reference(s)