Tg(Prop1-icre)517Sac
Transgene Detail

Summary

• Symbol: Tg(Prop1-icre)517Sac
• Name: transgene insertion 517, Sally A Camper
• MGI ID: MGI:5818681
• Synonyms: Prop1-cre, Tg(Prop1-cre)517Sac, Tg(Prop1-icre)1Sac
• Transgene: Tg(Prop1-icre)517Sac Location: unknown
• Alliance: Tg(Prop1-icre)517Sac page

Transgene origin

• Strain of Origin: C57BL/6J x (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Prop1-icre)517Sac expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Prop1 (MGI:109330)

• Mutation details: A BAC (RP23-479M11) containing the Prop1 gene was modified by inserting a cassette composed of the sequence encoding a mammalian codon usage optimized cre recombinase (icre), the bovine GH polyadenylation signal and an Frt-flanked kanamycin selection marker, into the exon 2 in frame. Flpe excision removed the kanamycin selection marker. The modified BAC was injected into pronuclei of fertilized oocytes to generate transgenic mice. Three lines, 432, 506, and 517 were genereted, and all 3 lines exhibit cre activity throughout the pituitary anterior lobe at 3 weeks of age. Line 517 was used for studies because it best reflected endogenous Prop1 expression. (J:233898)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Prop1 (mouse) Summary of all recombinase alleles driven by Prop1. Comparative matrix view of recombinase activities Alliance mouse Prop1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:233898 Davis SW, et al., All Hormone-Producing Cell Types of the Pituitary Intermediate and Anterior Lobes Derive From Prop1-Expressing Progenitors. Endocrinology. 2016 Apr;157(4):1385-96
• All: 4 reference(s)