Crh^tm2.2Maj
Targeted Allele Detail

Summary

• Symbol: Crh^tm2.2Maj
• Name: corticotropin releasing hormone; targeted mutation 2.2, Joseph Majzoub
• MGI ID: MGI:5823156
• Synonyms: Crh dl
• Gene: Crh Location: Chr3:19747565-19749560 bp, - strand Genetic Position: Chr3, 5.75 cM
• Alliance: Crh^tm2.2Maj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:238330
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A loxP site was introduced to intron 1 and a FRT-flanked neomycin cassette followed by a loxP site was placed in intron 2. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 2 floxed. Cre-mediated recombination removed exon 2. Immunohistochemistry of hypothalamus tissues confirmed that no protein was expressed in mutant mice. (J:238330)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crh Mutation: 42 strains or lines available

References

• Original: J:238330 Zhang R, et al., Loss of hypothalamic corticotropin-releasing hormone markedly reduces anxiety behaviors in mice. Mol Psychiatry. 2017 May;22(5):733-744
• All: 1 reference(s)