Hmcn1^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Hmcn1^em1(IMPC)J
• Name: hemicentin 1; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:5823446
• Synonyms: Hmcn1^em1J
• Gene: Hmcn1 Location: Chr1:150438275-150869186 bp, - strand Genetic Position: Chr1, 63.84 cM
• Alliance: Hmcn1^em1(IMPC)J page
• IMPC: Hmcn1 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele from project Hmcn1-8315J-M9720 was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences GCGTAATTCATTCTAGACAT, TGCTGAGTCTAGTCCTACAC, TAGCCAACAATTATATACTA and TATTTGAGTAATAGATCTTG, which resulted in a 436 bp deletion beginning at Chromosome 1 negative strand position 150,876,740 bp, TTGTGGCATTTCTGACTATG, and ending after TTTCTTTCCCCACCCATGTC at 150,876,305 bp (GRCm38/mm10). This mutation deletes exon 2 and 365 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 89 and early truncation 3 amino acids later. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Hmcn1 Mutation: 344 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)