Sp7^tm1.1Amc
Targeted Allele Detail

Summary

• Symbol: Sp7^tm1.1Amc
• Name: Sp7 transcription factor 7; targeted mutation 1.1, Andrew P McMahon
• MGI ID: MGI:5824213
• Synonyms: Sp7-BioFL, Sp7-Biotin-3XFLAG
• Gene: Sp7 Location: Chr15:102265038-102275498 bp, - strand Genetic Position: Chr15, 57.51 cM
• Alliance: Sp7^tm1.1Amc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:235520
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Insertion
• Mutation details: The targeting vector contains a loxP-flanked PGKneo cassette upstream of Biotin-3xFLAG (BioFL) sequence (encoding a biotin recognition motif and 3 copies of the FLAG epitope) inserted at the C-terminus of the Sp7 gene. Cre-mediated recombination removed the upstream floxed neo cassette. Western blotting detects the modified allele at slightly reduced levels as compared to wild-type. Temporal and spatial distributions is equivalent to the endogenous Sp7 gene expression. (J:235520)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sp7 Mutation: 21 strains or lines available

References

• Original: J:235520 Hojo H, et al., Sp7/Osterix Is Restricted to Bone-Forming Vertebrates where It Acts as a Dlx Co-factor in Osteoblast Specification. Dev Cell. 2016 May 9;37(3):238-53
• All: 1 reference(s)