Pdcd6ip^tm1.2Adz
Targeted Allele Detail

Summary

• Symbol: Pdcd6ip^tm1.2Adz
• Name: programmed cell death 6 interacting protein; targeted mutation 1.2, Alessandra d'Azzo
• MGI ID: MGI:5824749
• Synonyms: Alix^-
• Gene: Pdcd6ip Location: Chr9:113480812-113537327 bp, - strand Genetic Position: Chr9, 64.03 cM, cytoband F2
• Alliance: Pdcd6ip^tm1.2Adz page

Pdcd6ip^tm1.2Adz/Pdcd6ip^tm1.2Adz mice develop bilateral hydrocephalus

Show the 8 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:238436
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: One loxP site was inserted just upstream of exon 2 and a second loxP site 61 bp into exon 3. An FRT flanked neomycin resistance gene cassette was inserted into intron 1. Flp mediated recombination removed the neo cassette. The sequence representing exon 2 through most of exon 3 was deleted through cre-mediated recombination. Western blot analysis confirmed the absence of protein expression in brain, lung, liver, pancreas, and thymus lysates from homozygous mutant mice. (J:238436)

Generation of the Pdcd6ip^tm1.2Adz allele

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pdcd6ip Mutation: 42 strains or lines available

References

• Original: J:238436 Campos Y, et al., Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrier. Nat Commun. 2016 Jun 23;7:11876
• All: 1 reference(s)