Crkl^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Crkl^{tm1c(EUCOMM)Hmgu}
• Name: v-crk avian sarcoma virus CT10 oncogene homolog-like; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5825167
• Synonyms: Crkl^f, Crkl^f2
• Gene: Crkl Location: Chr16:17269851-17305298 bp, + strand Genetic Position: Chr16, 10.82 cM
• Alliance: Crkl^{tm1c(EUCOMM)Hmgu} page
• IMPC: Crkl gene page

Mutation origin

• Mutant Cell Line: HEPD0509_5_E11
• Germline Transmission: Earliest citation of germline transmission: J:239140
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 17286305 of Chromosome 16 upstream of exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 17287491. Exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele is created by flp recombinase expression in mice carrying this allele. (J:239140)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Crkl Mutation: 17 strains or lines available

References

• Original: J:239140 Lopez-Rivera E, et al., Genetic Drivers of Kidney Defects in the DiGeorge Syndrome. N Engl J Med. 2017 Feb 23;376(8):742-754
• All: 5 reference(s)