Spp1^tm1.1Dhd
Targeted Allele Detail

Summary

• Symbol: Spp1^tm1.1Dhd
• Name: secreted phosphoprotein 1; targeted mutation 1.1, Dick Heinegard
• MGI ID: MGI:5825176
• Synonyms: OPN^-
• Gene: Spp1 Location: Chr5:104582984-104588916 bp, + strand Genetic Position: Chr5, 50.68 cM
• Alliance: Spp1^tm1.1Dhd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:237104
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neomycin resistance cassette replaced exons 1 through 3. Cre-mediated recombination removed the selection cassette. Western blot analysis confirmed the absence of protein expression in the long bones. (J:237104)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spp1 Mutation: 43 strains or lines available

References

• Original: J:237104 Franzen A, et al., Altered osteoclast development and function in osteopontin deficient mice. J Orthop Res. 2008 May;26(5):721-8
• All: 3 reference(s)