Col1a1^{tm1(tetO-KMT2A/ELL)Dbry}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-KMT2A/ELL)Dbry}
• Name: collagen, type I, alpha 1; targeted mutation 1, David Bryder
• MGI ID: MGI:5825211
• Synonyms: Col1a1-tetO-MLL/ENL
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-KMT2A/ELL)Dbry} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:238261
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm1(tetO-KMT2A/ELL)Dbry} expresses 2 genes
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ELL (8178)

  Ell	(MGI:109377)

  inducible KMT2A/ELL fusion

  human

  KMT2A (4297)

  Kmt2a

  (MGI:96995)

  inducible KMT2A/ELL fusion

• Col1a1^{tm1(tetO-KMT2A/ELL)Dbry} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	tetO

• Mutation details: RMCE on KH2 cells replaced the FRT-flanked neomycin resistance cassette with one that contains a splice acceptor, polyadenylation sequence and the tetracycline promoter driving inducible expression of a human gene fusion associated with acute myeloid leukemia (MLL/ENL) followed by a polyadenylation sequence. (J:238261)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}. This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:238261 Ugale A, et al., Hematopoietic stem cells are intrinsically protected against MLL-ENL-mediated transformation. Cell Rep. 2014 Nov 20;9(4):1246-55
• All: 5 reference(s)