Gcg^{em1(cre/ERT2)Khk}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gcg^{em1(cre/ERT2)Khk}
• Name: glucagon; endonuclease-mediated mutation 1, Klaus H Kaestner
• MGI ID: MGI:5825448
• Synonyms: Gcg-CreERT2
• Gene: Gcg Location: Chr2:62304874-62313994 bp, - strand Genetic Position: Chr2, 35.85 cM
• Alliance: Gcg^{em1(cre/ERT2)Khk} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94077
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, No functional change, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Gcg^{em1(cre/ERT2)Khk} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gcg (MGI:95674)

• Mutation details: The allele is created using homologous recombination (aided by targeted CRISPR/cas9 endonuclease activity) to insert an IRES-Cre/ERT2 sequence into the 3' UTR of the gene. Cre expression is observed in pancreatic alpha cells and endocrine L cells. (J:94077, J:261359)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gcg (mouse) Summary of all recombinase alleles driven by Gcg. Alliance mouse Gcg gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gcg Mutation: 34 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 14 reference(s)