Cnn3^tm1.2Hjum
Targeted Allele Detail

Summary

• Symbol: Cnn3^tm1.2Hjum
• Name: calponin 3, acidic; targeted mutation 1.2, Hassan Jumaa
• MGI ID: MGI:5825459
• Synonyms: Cnn3 d
• Gene: Cnn3 Location: Chr3:121220190-121251854 bp, + strand Genetic Position: Chr3, 52.94 cM
• Alliance: Cnn3^tm1.2Hjum page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:237712
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 through 5 were replaced with a loxP site, cDNA encoding exons 2 through 7 with a GFP fusion, a polyadenylation sequence, FRT-flanked neomycin resistance cassette and a loxP site. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed the cDNA/GFP fusion. Western blot analysis confirmed the absence of protein expression in fetal brain tissue. (J:237712)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cnn3 Mutation: 79 strains or lines available

References

• Original: J:237712 Flemming A, et al., A Conditional Knockout Mouse Model Reveals That Calponin-3 Is Dispensable for Early B Cell Development. PLoS One. 2015;10(6):e0128385
• All: 2 reference(s)