Muc4^tm1Skb
Targeted Allele Detail

Summary

• Symbol: Muc4^tm1Skb
• Name: mucin 4; targeted mutation 1, Surinder K Batra
• MGI ID: MGI:5829375
• Gene: Muc4 Location: Chr16:32555089-32602786 bp, + strand Genetic Position: Chr16, 23.3 cM
• Alliance: Muc4^tm1Skb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:234205
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 9176 starting at position 32569030 and ending at position 32579762 of Chromosome 16 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon 2 through 6. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. RT-PCR confirmed the absence of transcript expression in the colon and lungs. (J:234205)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Muc4 Mutation: 238 strains or lines available

References

• Original: J:234205 Das S, et al., Mice deficient in Muc4 are resistant to experimental colitis and colitis-associated colorectal cancer. Oncogene. 2016 May 19;35(20):2645-54
• All: 2 reference(s)