Pik3r6^tm1.1Lste
Targeted Allele Detail

Summary

• Symbol: Pik3r6^tm1.1Lste
• Name: phosphoinositide-3-kinase regulatory subunit 5; targeted mutation 1.1, Len Stephens
• MGI ID: MGI:5829800
• Synonyms: p84^-
• Gene: Pik3r6 Location: Chr11:68393845-68443524 bp, + strand Genetic Position: Chr11, 41.76 cM
• Alliance: Pik3r6^tm1.1Lste page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:238900
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was obtained from the European Conditional Mouse Mutagenesis Program (EUCOMM) project. The targeting vector consisted of two homology arms, the 5 arm comprising exon 3 and the 3 arm comprising exons 5 to 8. Exon 4 was flanked by two loxP sequences, and its removal from the genome generates a shift in the open reading frame after splicing and an early termination of translation. The promoterless selection cassette present in the EUCOMM vector was replaced by two elements present in the pACN vector: a neomycin resistance gene under the control of a PGK (phosphoglycerate kinase) promoter and a self-excising Cre recombinase cassette under the control of a testis-specific angiotensin-converting enzyme (tACE) promoter. Because both elements are flanked by loxP sites, this construct enabled the cassette and exon 4 to be deleted in the testis of the male chimeras, producing heterozygous p84+/ germline cells. (J:238900)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pik3r6 Mutation: 34 strains or lines available

References

• Original: J:238900 Deladeriere A, et al., The regulatory subunits of PI3Kgamma control distinct neutrophil responses. Sci Signal. 2015 Jan 20;8(360):ra8
• All: 1 reference(s)