Mcoln2^{tm1e.1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mcoln2^{tm1e.1(KOMP)Wtsi}
• Name: mucolipin 2; targeted mutation 1e.1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5881907
• Synonyms: TRPML2 KO
• Gene: Mcoln2 Location: Chr3:145855588-145901268 bp, + strand Genetic Position: Chr3, 71.03 cM
• Alliance: Mcoln2^{tm1e.1(KOMP)Wtsi} page
• IMPC: Mcoln2 gene page

Mutation origin

• Mutant Cell Line: EPD0300_5_B06
• Germline Transmission: Earliest citation of germline transmission: J:239875
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 145877259 of Chromosome 3 upstream of the critical exon (exon 4) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression removed the neomycin selection cassette. Western blot analysis using rabbit anti-MCOLN2 antibody confirmed the absence of protein in bone marrow-derived macrophages (BMDMs) isolated from homozygous mutant mice both under resting conditions and upon BMDM activation with LPS. (J:239875)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mcoln2 Mutation: 36 strains or lines available

References

• Original: J:239875 Sun L, et al., Novel Role of TRPML2 in the Regulation of the Innate Immune Response. J Immunol. 2015 Nov 15;195(10):4922-32
• All: 2 reference(s)