Slc13a4^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Slc13a4^{tm1d(EUCOMM)Wtsi}
• Name: solute carrier family 13 (sodium/sulfate symporters), member 4; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5881938
• Synonyms: Slc13a4^-
• Gene: Slc13a4 Location: Chr6:35244888-35285061 bp, - strand Genetic Position: Chr6, 15.26 cM
• Alliance: Slc13a4^{tm1d(EUCOMM)Wtsi} page
• IMPC: Slc13a4 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:239786
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 35266501 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 35267312. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). (J:239786)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc13a4 Mutation: 34 strains or lines available

References

• Original: J:239786 Rakoczy J, et al., Loss of the sulfate transporter Slc13a4 in placenta causes severe fetal abnormalities and death in mice. Cell Res. 2015 Nov;25(11):1273-6
• All: 2 reference(s)