Ube3a^tm1.1Bdph
Targeted Allele Detail

Summary

• Symbol: Ube3a^tm1.1Bdph
• Name: ubiquitin protein ligase E3A; targeted mutation 1.1, Benjamin D Philpot
• MGI ID: MGI:5882092
• Synonyms: Ube3a^FLOX
• Gene: Ube3a Location: Chr7:58878498-58961284 bp, + strand Genetic Position: Chr7, 33.95 cM
• Alliance: Ube3a^tm1.1Bdph page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:235694
• Parent Cell Line: PRX-B6N (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A conditional ready (floxed) allele was created by removing the FRT-flanked lacZ sequence, loxP site, neomycin cassette under the control of the human beta-actin promoter and SV40 polyA via flp-mediated recombination from the KO first allele generated using EUCOMM vector (PG00126_Z_3_B08), leaving exon 5 flanked by loxP sites. (J:227476, J:235694)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ube3a Mutation: 68 strains or lines available

References

• Original: J:235694 Berrios J, et al., Loss of UBE3A from TH-expressing neurons suppresses GABA co-release and enhances VTA-NAc optical self-stimulation. Nat Commun. 2016;7:10702
• All: 7 reference(s)