Cpeb2^tm1.2Yshu
Targeted Allele Detail

Summary

• Symbol: Cpeb2^tm1.2Yshu
• Name: cytoplasmic polyadenylation element binding protein 2; targeted mutation 1.2, Yi-Shuian Huang
• MGI ID: MGI:5882111
• Synonyms: Cpeb2-KO, Cpeb2^KO
• Gene: Cpeb2 Location: Chr5:43390513-43447067 bp, + strand Genetic Position: Chr5, 23.65 cM, cytoband B
• Alliance: Cpeb2^tm1.2Yshu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:237513
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A single loxP site is inserted 3' to exon 5. An Frt-PGK-Neo-Frt-loxP cassette was inserted to the 5'-end of exon 3. The neo cassette was removed by crossing to mice carrying the Frt recombinase driven by the beta-actin promoter, leaving single loxP sites flanking exons 3-5. Cre-mediated recombination resulted in excision of exons 3-5. Western blot analysis confirmed the absence of protein in homozygous mutant tissues. (J:237513)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cpeb2 Mutation: 35 strains or lines available

References

• Original: J:237513 Lai YT, et al., Deficiency of CPEB2-Confined Choline Acetyltransferase Expression in the Dorsal Motor Nucleus of Vagus Causes Hyperactivated Parasympathetic Signaling-Associated Bronchoconstriction. J Neurosci. 2016 Dec 14;36(50):12661-12676
• All: 3 reference(s)