Wbp2nl^tm1.1Osb
Targeted Allele Detail

Summary

• Symbol: Wbp2nl^tm1.1Osb
• Name: WBP2 N-terminal like; targeted mutation 1.1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:5882390
• Synonyms: Wbp2nl^{tm1b(EUCOMM)Osb}
• Gene: Wbp2nl Location: Chr15:82183155-82198824 bp, + strand Genetic Position: Chr15, 38.56 cM, cytoband E2
• Alliance: Wbp2nl^tm1.1Osb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:227499
• Parent Cell Line: EGR-G101 (ES Cell)
• Strain of Origin: C57BL/6NSlc-Tg(CAG-EGFP,Acr-EGFP)2Osb

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 82189400 of Chromosome 15 upstream of the critical exons 2 through 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 2 through 4 at position 82191234. The critical exon 2 through 4 are thus flanked by loxP sites. Cre-mediated recombination removed the selection cassette and exons 2 through 4. Western blot analysis confirmed the absence of protein expression in testes. (J:227499)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Wbp2nl Mutation: 30 strains or lines available

References

• Original: J:227499 Satouh Y, et al., Sperm postacrosomal WW domain-binding protein is not required for mouse egg activation. Biol Reprod. 2015 Oct;93(4):94
• All: 1 reference(s)