Cpne6^tm1.1Rueg
Targeted Allele Detail

Summary

• Symbol: Cpne6^tm1.1Rueg
• Name: copine VI; targeted mutation 1.1, Markus Ruegg
• MGI ID: MGI:5882517
• Synonyms: Cpne6^nls-LacZ
• Gene: Cpne6 Location: Chr14:55747902-55754888 bp, + strand Genetic Position: Chr14, 28.19 cM
• Alliance: Cpne6^tm1.1Rueg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:239922
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6 x 129

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The entire complement of coding exons 2-16 was replaced with an nls-lacZ reporter gene cassette and a loxP-flanked neomycin resistance gene cassette. Subsequently, the neo cassette was removed through cre-mediated recombination, leaving a reporter and null allele. Western blot analysis of hippocampal lysates confirmed the absence of protein expression in homozygous mutant mice. (J:239922)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cpne6 Mutation: 44 strains or lines available

Notes

The embryonic stem cells used in the original reference (J:239922) were "75% C57BL/6, 25% 129Sv".

References

• Original: J:239922 Reinhard JR, et al., The calcium sensor Copine-6 regulates spine structural plasticity and learning and memory. Nat Commun. 2016 May 19;7:11613
• All: 1 reference(s)