Pgam5^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pgam5^{tm1d(EUCOMM)Wtsi}
• Name: phosphoglycerate mutase family member 5; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5882561
• Synonyms: Pgam5^-
• Gene: Pgam5 Location: Chr5:110407001-110417765 bp, - strand Genetic Position: Chr5, 53.43 cM, cytoband F
• Alliance: Pgam5^{tm1d(EUCOMM)Wtsi} page
• IMPC: Pgam5 gene page

Mutation origin

• Mutant Cell Line: EPD0226_5_H02
• Germline Transmission: Earliest citation of germline transmission: J:240016
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 110414719 of Chromosome 5 upstream of the critical exon (exon 2) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 110415572. Exon 2 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and exon 2. Western blot analysis confirmed the absence of protein expression in the liver, colon and spleen tissues from homozygous mutant mice. (J:240016)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pgam5 Mutation: 26 strains or lines available

References

• Original: J:240016 Moriwaki K, et al., The Mitochondrial Phosphatase PGAM5 Is Dispensable for Necroptosis but Promotes Inflammasome Activation in Macrophages. J Immunol. 2016 Jan 01;196(1):407-15
• All: 3 reference(s)