Smc5^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Smc5^{tm1c(KOMP)Wtsi}
• Name: structural maintenance of chromosomes 5; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5882565
• Synonyms: Smc5^flox
• Gene: Smc5 Location: Chr19:23183815-23251261 bp, - strand Genetic Position: Chr19, 18.04 cM
• Alliance: Smc5^{tm1c(KOMP)Wtsi} page
• IMPC: Smc5 gene page

Mutation origin

• Mutant Cell Line: EPD0395_1_F05
• Germline Transmission: Earliest citation of germline transmission: J:240155
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 23240733 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 23241614. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:240155)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smc5 Mutation: 42 strains or lines available

References

• Original: J:240155 Pryzhkova MV, et al., Conditional mutation of Smc5 in mouse embryonic stem cells perturbs condensin localization and mitotic progression. J Cell Sci. 2016 Apr 15;129(8):1619-34
• All: 5 reference(s)