Podxl2^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Podxl2^{tm1c(EUCOMM)Wtsi}
• Name: podocalyxin-like 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5883834
• Synonyms: EG^flox, Podxl2^flox
• Gene: Podxl2 Location: Chr6:88819540-88852026 bp, - strand Genetic Position: Chr6, 39.6 cM
• Alliance: Podxl2^{tm1c(EUCOMM)Wtsi} page
• IMPC: Podxl2 gene page

Mutation origin

• Mutant Cell Line: EPD0647_7_C06
• Germline Transmission: Earliest citation of germline transmission: J:240523
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 88825445 of Chromosome 6 upstream of the critical exons 3 and 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons at position 88827396. The critical exons 3 and 4 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exons 3 and 4. (J:240523)

Expression

In Mice Carrying this Mutation:

30 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Podxl2 Mutation: 26 strains or lines available

References

• Original: J:240523 Yang Z, et al., Role of CD34 family members in lumen formation in the developing kidney. Dev Biol. 2016 Oct 01;418(1):66-74
• All: 1 reference(s)