Tgfbi^tm1.1Nap
Targeted Allele Detail

Summary

• Symbol: Tgfbi^tm1.1Nap
• Name: transforming growth factor, beta induced; targeted mutation 1.1, Nathalie Allaman-Pillet
• MGI ID: MGI:5888672
• Synonyms: Bigh3^-
• Gene: Tgfbi Location: Chr13:56757399-56787172 bp, + strand Genetic Position: Chr13, 30.09 cM, cytoband B-C1
• Alliance: Tgfbi^tm1.1Nap page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:230752
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 1. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 1. Cre-mediated recombination removed the selection cassette and exon 1. Western blotting and immunohistochemistry confirmed the absence of protein (even of a smaller size) in the cornea of homozygous mutant mice. (J:230754)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tgfbi Mutation: 34 strains or lines available

References

• Original: J:230754 Allaman-Pillet N, et al., Tgfbi/Bigh3 silencing activates ERK in mouse retina. Exp Eye Res. 2015 Nov;140:159-70
• All: 3 reference(s)