Usp21^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Usp21^{tm1c(EUCOMM)Wtsi}
• Name: ubiquitin specific peptidase 21; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5888903
• Synonyms: Usp21^flox, Usp21^Loxp
• Gene: Usp21 Location: Chr1:171109523-171115534 bp, - strand Genetic Position: Chr1, 79.3 cM
• Alliance: Usp21^{tm1c(EUCOMM)Wtsi} page
• IMPC: Usp21 gene page

Mutation origin

• Mutant Cell Line: EPD0716_10_H09
• Germline Transmission: Earliest citation of germline transmission: J:240648
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 171111927 of Chromosome 1 upstream of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 6 at position 171113776. Exons 3-6 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exons 3-6. (J:240648)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Usp21 Mutation: 25 strains or lines available

References

• Original: J:240648 Li Y, et al., USP21 prevents the generation of T-helper-1-like Treg cells. Nat Commun. 2016 Nov 18;7:13559
• All: 4 reference(s)