Tmem258^{tm1.1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Tmem258^{tm1.1(KOMP)Vlcg}
• Name: transmembrane protein 258; targeted mutation 1.1, Velocigene
• MGI ID: MGI:5892142
• Synonyms: Tmem258^-
• Gene: Tmem258 Location: Chr19:10181566-10185188 bp, + strand Genetic Position: Chr19, 6.54 cM
• Alliance: Tmem258^{tm1.1(KOMP)Vlcg} page
• IMPC: Tmem258 gene page

Mutation origin

• Mutant Cell Line: 19703A-G5
• Germline Transmission: Earliest citation of germline transmission: J:240635
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 3026bp between positions 10278755-10281780 of Chromosome 19 (Genome Build37). Exons 2 and 3 were replaced with a lacZ gene and a loxP-flanked neomycin selection cassette. The loxP-flanked cassette was removed by cre-mediated recombination. (J:240635)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tmem258 Mutation: 13 strains or lines available

References

• Original: J:240635 Graham DB, et al., TMEM258 Is a Component of the Oligosaccharyltransferase Complex Controlling ER Stress and Intestinal Inflammation. Cell Rep. 2016 Dec 13;17(11):2955-2965
• All: 1 reference(s)