Mcmdc2^{tm3b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Mcmdc2^{tm3b(EUCOMM)Hmgu}
• Name: minichromosome maintenance domain containing 2; targeted mutation 3b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5892145
• Synonyms: Mcmdc2^{insertion-deletion}
• Gene: Mcmdc2 Location: Chr1:9978863-10011179 bp, + strand Genetic Position: Chr1, 2.19 cM
• Alliance: Mcmdc2^{tm3b(EUCOMM)Hmgu} page
• IMPC: Mcmdc2 gene page

Mutation origin

• Mutant Cell Line: HEPD0800_2_F07
• Germline Transmission: Earliest citation of germline transmission: J:236226
• Parent Cell Line: JM8A3.N1.C2 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 9984471 of Chromosome 1 upstream of exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 7 at position 9987378. Exons 5-7 are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked exons 5-7. (J:236226)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mcmdc2 Mutation: 32 strains or lines available

References

• Original: J:236226 Finsterbusch F, et al., Alignment of Homologous Chromosomes and Effective Repair of Programmed DNA Double-Strand Breaks during Mouse Meiosis Require the Minichromosome Maintenance Domain Containing 2 (MCMDC2) Protein. PLoS Genet. 2016 Oct;12(10):e1006393
• All: 1 reference(s)