Anks1b^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Anks1b^{tm1b(KOMP)Wtsi}
• Name: ankyrin repeat and sterile alpha motif domain containing 1B; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5896631
• Gene: Anks1b Location: Chr10:89709371-90809162 bp, + strand Genetic Position: Chr10, 45.28 cM
• Alliance: Anks1b^{tm1b(KOMP)Wtsi} page
• IMPC: Anks1b gene page

Mutation origin

• Mutant Cell Line: EPD0613_3_C08
• Germline Transmission: Earliest citation of germline transmission: J:240601
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 90516200 of Chromosome 10 upstream of exon 17 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 17 at position 90517051. Exon 17 is thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked exon 17. Western blot analysis of cortical tissue lysates from homozygous mice confirmed the absence of protein expression. (J:240601)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Anks1b Mutation: 54 strains or lines available

References

• Original: J:240601 Enga RM, et al., Initial characterization of behavior and ketamine response in a mouse knockout of the post-synaptic effector gene Anks1b. Neurosci Lett. 2017 Feb 22;641:26-32
• All: 2 reference(s)