Spoptm1d(KOMP)Mbp
Targeted Allele Detail
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Symbol: |
Spoptm1d(KOMP)Mbp |
Name: |
speckle-type BTB/POZ protein; targeted mutation 1d, Mouse Biology Program, UC Davis |
MGI ID: |
MGI:5896729 |
Synonyms: |
SpopdeltaEx |
Gene: |
Spop Location: Chr11:95304906-95384232 bp, + strand Genetic Position: Chr11, 59.01 cM
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Alliance: |
Spoptm1d(KOMP)Mbp page
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Germline Transmission: |
Earliest citation of germline transmission:
J:239074
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Parent Cell Line: |
JM8A3.N1 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Allele Type: |
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Targeted (Null/knockout) |
Mutations: |
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Insertion, Intragenic deletion
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Mutation details: The L1L2_Bact_P cassette was inserted at position 95364610 of Chromosome 11 upstream of the exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 5 at position 95365683. Exons 4 and 5 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Absence of protein expression was confirmed by immunoblot analysis. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml.
(J:239074)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Spop Mutation: |
36 strains or lines available
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Original: |
J:239074 Cai H, et al., Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling. Proc Natl Acad Sci U S A. 2016 Dec 20;113(51):14751-14756 |
All: |
3 reference(s) |
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