Spop^{tm1d(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Spop^{tm1d(KOMP)Mbp}
• Name: speckle-type BTB/POZ protein; targeted mutation 1d, Mouse Biology Program, UC Davis
• MGI ID: MGI:5896729
• Synonyms: Spop^deltaEx
• Gene: Spop Location: Chr11:95304906-95384232 bp, + strand Genetic Position: Chr11, 59.01 cM
• Alliance: Spop^{tm1d(KOMP)Mbp} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:239074
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 95364610 of Chromosome 11 upstream of the exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 5 at position 95365683. Exons 4 and 5 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Absence of protein expression was confirmed by immunoblot analysis. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:239074)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spop Mutation: 35 strains or lines available

References

• Original: J:239074 Cai H, et al., Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling. Proc Natl Acad Sci U S A. 2016 Dec 20;113(51):14751-14756
• All: 3 reference(s)