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Spoptm1d(KOMP)Mbp
Targeted Allele Detail
Summary
Symbol: Spoptm1d(KOMP)Mbp
Name: speckle-type BTB/POZ protein; targeted mutation 1d, Mouse Biology Program, UC Davis
MGI ID: MGI:5896729
Synonyms: SpopdeltaEx
Gene: Spop  Location: Chr11:95304906-95384232 bp, + strand  Genetic Position: Chr11, 59.01 cM
Alliance: Spoptm1d(KOMP)Mbp page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:239074
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 95364610 of Chromosome 11 upstream of the exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 5 at position 95365683. Exons 4 and 5 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Absence of protein expression was confirmed by immunoblot analysis. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:239074)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Spop Mutation:  36 strains or lines available
References
Original:  J:239074 Cai H, et al., Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling. Proc Natl Acad Sci U S A. 2016 Dec 20;113(51):14751-14756
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory