Tg(CAG-GFP,-EIF4EBP1*)2969Als
Transgene Detail

Summary

• Symbol: Tg(CAG-GFP,-EIF4EBP1*)2969Als
• Name: transgene insertion 2969, Albert La Spada
• MGI ID: MGI:5896791
• Synonyms: 4EBP1mt, Tg-4EBP1mt
• Transgene: Tg(CAG-GFP,-EIF4EBP1*)2969Als Location: unknown
• Alliance: Tg(CAG-GFP,-EIF4EBP1*)2969Als page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(CAG-GFP,-EIF4EBP1*)2969Als expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  EIF4EBP1 (1978)

  Eif4ebp1

  (MGI:103267)

  contains T37A and T46A substitutions

• Mutation details: The transgene has (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from CAGGS), a loxP-GFP-3xSTOP-loxP cassette and a cDNA sequence encoding human EIF4EBP1 rendered constitutively active and mTORC1-nonresponsive by threonine-to-alanine mutations at two mTOR phosphorylation sites [T37A and T46A]). In the absence of Cre recombinase, mice have widespread expression of GFP, and no human EIF4EBP1 expression. (J:224536)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:224536 Tsai S, et al., Muscle-specific 4E-BP1 signaling activation improves metabolic parameters during aging and obesity. J Clin Invest. 2015 Aug 3;125(8):2952-64
• All: 4 reference(s)