Ddrgk1^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ddrgk1^{tm1c(EUCOMM)Hmgu}
• Name: DDRGK domain containing 1; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5896836
• Synonyms: UFBP1^F
• Gene: Ddrgk1 Location: Chr2:130495955-130506549 bp, - strand Genetic Position: Chr2, 63.24 cM, cytoband F3
• Alliance: Ddrgk1^{tm1c(EUCOMM)Hmgu} page
• IMPC: Ddrgk1 gene page

Mutation origin

• Mutant Cell Line: HEPD0618_2_D02
• Germline Transmission: Earliest citation of germline transmission: J:231701
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 130503397 of Chromosome 2 upstream of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4 at position 130504550. Exons 3 and 4 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exons 3 and 4. (J:231701)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ddrgk1 Mutation: 21 strains or lines available

References

• Original: J:231701 Cai Y, et al., UFBP1, a Key Component of the Ufm1 Conjugation System, Is Essential for Ufmylation-Mediated Regulation of Erythroid Development. PLoS Genet. 2015 Nov;11(11):e1005643
• All: 4 reference(s)