Tm6sf2^em1Yech
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tm6sf2^em1Yech
• Name: transmembrane 6 superfamily member 2; endonuclease-mediated mutation 1, Y Eugene Chen
• MGI ID: MGI:5896993
• Synonyms: Tm6sf2 KO
• Gene: Tm6sf2 Location: Chr8:70525574-70532716 bp, + strand Genetic Position: Chr8, 34.15 cM
• Alliance: Tm6sf2^em1Yech page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Insertion
• Mutation details: CRISPR/Cas9 mutagenesis was used to create a mutation of a C/G base pair inserted into the coding region right after start codon, disrupting the open reading frame. The guide RNA targeted the coding region sequence 5'-GACATCCCGCCGC-3'. Western blot analysis confirmed that the expression of the encoded protein was ablated. (J:235704)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tm6sf2 Mutation: 32 strains or lines available

References

• Original: J:235704 Fan Y, et al., Hepatic Transmembrane 6 Superfamily Member 2 Regulates Cholesterol Metabolism in Mice. Gastroenterology. 2016 May;150(5):1208-18
• All: 1 reference(s)