Memo1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Memo1^{tm1c(EUCOMM)Wtsi}
• Name: mediator of cell motility 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5896997
• Synonyms: Memo1^flp
• Gene: Memo1 Location: Chr17:74506031-74602516 bp, - strand Genetic Position: Chr17, 45.63 cM, cytoband E2
• Alliance: Memo1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Memo1 gene page

Mutation origin

• Mutant Cell Line: EPD0785_3_A06
• Germline Transmission: Earliest citation of germline transmission: J:233609
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 74551788 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 74552564. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:233609)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Memo1 Mutation: 47 strains or lines available

References

• Original: J:233609 Van Otterloo E, et al., MEMO1 drives cranial endochondral ossification and palatogenesis. Dev Biol. 2016 Jul 15;415(2):278-95
• All: 1 reference(s)