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Opn3tm2b(EUCOMM)Wtsi
Targeted Allele Detail
Summary
Symbol: Opn3tm2b(EUCOMM)Wtsi
Name: opsin 3; targeted mutation 2b, Wellcome Trust Sanger Institute
MGI ID: MGI:5897190
Synonyms: Opn3lacz
Gene: Opn3  Location: Chr1:175489993-175520198 bp, - strand  Genetic Position: Chr1, 81.66 cM, cytoband H3
Alliance: Opn3tm2b(EUCOMM)Wtsi page
IMPC: Opn3 gene page
Mutation
origin
Mutant Cell Line:  EPD0197_3_E01
Germline Transmission:  Earliest citation of germline transmission: J:227078
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 175492614 of Chromosome 1 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 175493673. The critical exon 2 is thus flanked by loxP sites. Cre expression occur without flp expression generates a reporter knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:227078)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Opn3 Mutation:  25 strains or lines available
References
Original:  J:227078 Buhr ED, et al., Neuropsin (OPN5)-mediated photoentrainment of local circadian oscillators in mammalian retina and cornea. Proc Natl Acad Sci U S A. 2015 Oct 20;112(42):13093-8
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory